General Cell Based Assays
Eurofins Discoverx PathHunter eXpress CCR7 HEK 293 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0195E1CP0M)
The PathHunter CCR7 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CCL19/MIP-3β) based activation of CCR7 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CCR7 and EA-tagged β-Arrestin-2. Activation of CCR7 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
ABclonal Technology HRP-conjugated B-Actin Mouse
This gene encodes one of six different actin proteins Actins are highly conserved proteins that are involved in cell motility structure integrity and intercellular signaling The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed Mutations in this gene cause Baraitser-Winter syndrome 1 which is characterized by intellectual disability with a distinctive facial appearance in human patients Numerous pseudogenes of this gene have been identified throughout the human genome
Cayman Chemical D-Fructose-1 6-bIsphosphate 1g
An intermediate in carbohydrate metabolism, including glycolysis and gluconeogenesis
Eurofins Discoverx cAMP Hunter GPBAR1 Bioassay Kit, 2-Plate. (95-0049Y2-00139)
The cAMP Hunter CHO-K1 GPBAR1 Bioassay Kit provides a robust and highly sensitive functional, cell-based assay to measure drug potency and detect neutralizing antibodies. The bioassay kit contains all the materials needed to run the assay, including cryopreserved cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The ready-to-use cryopreserved cells have been manufactured to ensure long-term assay reproducibility, and faster implementation from characterization to QC lot release. This bioassay has been optimized for a 96-well format. The protocol can be optimized further for running the assay in a high-throughput 384-well format. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx AssayComplete Cell Plating 15 Reagent, 100. (93-0563R15A)
AssayComplete Cell Plating 15 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 100 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter eXpress mEDNRB CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0602E2MCP0L)
The PathHunter mEDNRB eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. endothelin-3) based activation of mEDNRB GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mEDNRB and EA-tagged β-Arrestin-2. Activation of mEDNRB stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx AssayComplete Cell Plating 13 Reagent, 500. (93-0563R13B)
AssayComplete Cell Plating 13 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Abcam Cerivastatin sodium, HMG-CoA reductase inhibitor, 10MG
MW 481.5 Da, Purity >98%. Potent HMG-CoA reductase inhibitor (Ki = 13 nM). Shows higher potency than Lovastatin (ab120614). Enantiomerically pure pyridine derivative. Reduces plasma cholesterol levels in vivo. Orally active.
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress MTNR1B CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0630E2CP0L)
The PathHunter MTNR1B eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. melatonin) based activation of MTNR1B GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MTNR1B and EA-tagged β-Arrestin-2. Activation of MTNR1B stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx InCELL Hunter eXpress Bcl2L1-Bax Protein Binding Assay, 10-plate Kit. (96-0009E3CP16L)
The InCELL Hunter eXpress Bcl2L1-Bax Protein Binding Assay provides an easy-to-use cell based assay to study small molecule drug candidates that disrupt protein binding. This kit assesses compound-based disruption of Bcl2L1-Bax binding via detection of Bax levels. This product uses EFC technology. The cell line included overexpresses Bcl2L1 target in an untagged form and Bax binding partner fused to ProLabel. When a compound engages the Bcl2L1 target protein, partner binding is disrupted and the destabilized Bax is degraded, leading to a loss of EFC signal upon addition of EA containing detection reagent. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
STEMCELL Technologies Wheat Germ Agglutinin WGA i
Fluorescent dye conjugates for staining cell membranes of gram-positive bacteria, mammalian cells, and yeast bud scars - 1000 Ug
Cayman Chemical Glutamyl Trnsferas Assay Kit
Cayman’s GGT Activity Assay Kit provides a convenient method of detecting GGT activity in plasma, serum, and tissue lysates. In this assay, GGT catalyzes the transfer of the γ-glutamyl group from L-γ-glutamyl-p-nitroaniline (γGpNA) to glycylglycine resulting in the liberation of the colored product p-nitroaniline (pNA), which can be measured at 418 nm. Under circumstances in which the GGT activity rate is limiting, the rate of increase is directly proportional to the GGT activity in the sample.
Eurofins Discoverx PathHunter MRGPRX2 Bioassay Kit, 10-Plate. (93-0309Y2-00144)
The PathHunter MRGPRX2 Bioassay kit provides a robust, highly sensitive, and easy-to-use functional cell based assay to measure drug potency and detect neutralizing antibodies. The bioassay kit contains all the materials needed for a complete assay, including cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The ready-to-use cryopreserved cells have been manufactured to ensure assay reproducibility, and faster implementation from characterization to lot release. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.